Tissue Engineering Laboratory | Ft.Tul.cz

Tissue Engineering Laboratory
The laboratory focuses on the biological testing of developed (nano)materials with cell cultures. The primary aspects studied in tested materials include cytotoxicity, cell adhesion, and proliferation on the material. These materials have the potential to be used as tissue scaffolds in various biomedical applications. The laboratory primarily utilizes mouse fibroblasts, human dermal fibroblasts, keratinocytes, vascular endothelial cells, osteoblasts, and others.
Location:	Building E, Basement
Contact Person:	Ing. Senta Műllerová Ing. Markéta Klíčová RNDr. Jana Horáková, Ph.D.
Phone:	+420 48 535 6471

Laboratory Equipment
TECAN The Spark Spectrophotometer	This device is primarily used for assessing the metabolic activity of cells on materials (e.g., MTT test). It is also utilized for measuring the incorporation of bioactive substances into scaffolds. CO2 Incubators
Telstar BioAir Laminar Flow Hoods	Class II biological safety cabinets used for working under sterile conditions.
CO₂ Incubator Satorium N-Biotech	These devices are used for cell culture at the required temperature and carbon dioxide concentration.
Ultra-Low Freeze (-80 °C)	Used for long-term storage of cell cultures.
Fluorescence Microscope Nikon Eclipse Ti
Other	Inverted microscope, CMOS camera, water bath, Luna Cell Counter, PCR cycler, DNA electrophoresis apparatus, autoclave, centrifuge, fluorimeter, vertical shaker with thermostat, and Dewar flask for liquid nitrogen (for storing cell cultures).